《植物生理学报》 2015, 51(8): 1331-1338

不同浓度Cd²⁺胁迫对苎麻叶绿素及其光合荧光特性的影响

简敏菲^1,2,*, 杨叶萍¹, 余厚平², 龚秋林³, 陈勇玲³

江西师范大学¹生命科学学院, 江西省亚热带植物资源保护与利用重点实验室; ²鄱阳湖湿地与流域研究教育部重点实验室, 南昌330022; ³江西省宜春市农业科学研究所, 江西宜春336000

通信作者：简敏菲；E-mail: jianminfei0914@163.com；Tel: 0791-88120193

摘　要：

以苎麻为材料, 采用盆栽培养, 测定不同浓度镉胁迫下苎麻叶片中叶绿素含量、光合荧光参数及其荧光成像的变化, 以及苎麻的镉耐受性及其光合荧光特性的关系。结果表明, 短时间镉胁迫下, 苎麻叶片的叶绿素a (Chla)、叶绿素b (Chlb)、总叶绿素含量(Chlt)、净光合速率(P_n)、气孔导度(G_s)、蒸腾速率(T_r)等指标与对照相比均呈上升趋势, 而胞间CO₂浓度(C_i)与稳态下的非光化学淬灭(NPQl_ss)呈下降趋势, 且P_n、G_s、T_r与PSII最大量子产率(QY_max)均在Cd²⁺浓度为60 mg•L^-1胁迫下出现最大值; 长时间镉胁迫下, 苎麻叶片的Chla、Chlb、Chlt、P_n、G_s、T_r等指标与对照相比则均呈下降趋势, 而C_i呈上升趋势, 除 240 mg•L^-1 Cd²⁺胁迫后的苎麻叶QY_max与NPQl_ss值分别高出对照的2.5%和75.9%外, 其余不同镉浓度处理后的苎麻叶QY_max或NPQl_ss值均低于对照。

关键词：苎麻; 镉胁迫; 光合特性; 荧光成像; 耐受性

收稿：2015-03-09   修定：2015-07-11

资助：国家自然科学基金项目(41161035和41461042)、国家科技部“十二五”科技支撑计划资助项目(2011BAC13B02)、江西省自然科学基金项目(20142BAB204007)和江西省重大生态安全问题监控协同创新中心资助项目(JXS-EW-03)。

Influences of Different Cadmium Concentration Stress on Chlorophyll and Its Photosynthetic Fluorescence Characteristics of Ramie (Boehmeria nivea)

JIAN Min-Fei^1,2,*, YANG Ye-Ping¹, YU Hou-Ping², GONG Qiu-Lin³, CHEN Yong-Ling³

¹College of Life Sciences, Jiangxi Provincial Key Lab of Protection and Utilization of Subtropical Plant Resources; ²Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China; ³Yichun Agricultural Science Research Institute of Jiangxi Province, Yichun, Jiangxi 336000, China

Corresponding author: JIAN Min-Fei; E-mail: jianminfei0914@163.com; Tel: 0791-88120193

Abstract:

On the basis of pot experiment methods, we selected ramie (Boehmeria nivea) as the materials, and measured all contents of chlorophyll, photosynthetic fluorescence parameters and fluorescence imaging in order to study the relationships among the tolerance to cadmium contamination of B. nivea and its photosynthetic fluorescence characteristics under different concentrations of cadmium stress. Under different concentrations of cadmium stress in the short time of 21 days, the results showed that the values of the most parameters of B. nivea leaves increased more than those of the control group without any heavy metal stress, and the main parameters which included chlorophyll a, chlorophyll b, the total chlorophyll, and the net photosynthetic rate (P_n), stomatal conductance (G_s), transpiration rate (T_r) and etc., while the values of other parameters included the intercellular CO₂ concentration (C_i) and the non-photochemical quenching under stable state (NPQl_ss) decreased less than those of the control group, and the values of the parameters included the net photosynthetic rate (P_n), transpiration rate (T_r), stomatal conductance (G_s) and the maximum quantum yield (QY_max) presented the highest under 60 mg•L^-1 cadmium stress. With the increasing of stress time, under different concentrations of cadmium stress in the long time of 49 days, the values of chlorophyll a, chlorophyll b, total chlorophyll contents, P_n, G_s, T_r of B. nivea leaves decreased than those of the control group, while the values of C_i increased. And the change trend showed that the values of QY_max and NPQl_ss were lower than those of the control group under different concentrations of cadmium stress except the 240 mg•L^-1 cadmium, and which values were higher than those of the control group 2.5%, 75.9%.

Key words: ramie (Boehmeria nivea); cadmium stress; photosynthetic characteristics; chlorophyll fluorescence imaging; tolerance